The amphibian retina contains a reservoir of active neural stem cells in a defined niche, localized at the margin of the retina, that contribute to persistent eye growth throughout life, as well as to regeneration following retinal damage. This system has proved to be very powerful to study in vivo the molecular mechanisms underlying neural stem cell behaviour. We focussed our interest on the role of various signalling pathways (Wnt, Hedgehog and Hippo) in the regulation of the proliferative activity of these cells. We investigated in particular their impact on several cellular parameters such as the cell cycle kinetics and the timing of cell cycle exit. Altogether, our data highlight how these signalling pathways interact to regulate retinal stem cell activity and thereby modulate the growth of the post-embryonic retina.